L1 CNS (Larva1099) eFIB-SEM CATMAID Data Viewer

Access

The Larva1099 CATMAID instance is hosted and maintained by Virtual Fly Brain (VFB) as part of its mission to integrate and preserve key Drosophila neuroscience datasets. The instance is accessible at: https://larva1099.catmaid.virtualflybrain.org/

This is a distinct volume from the L1EM serial-section TEM dataset also hosted by VFB: it is a separate first instar larva (sample 1099) imaged by enhanced focused-ion-beam scanning electron microscopy (eFIB-SEM) at 12 × 12 × 12 nm/voxel isotropic resolution.

Source Publication

The volume and reconstructions are from:

• Randel N, Wang C, Clayton MS, Wang K, Pang S, Xu CS, Champion A, Hess HF, Cardona A, Keller PJ, Zlatic M (2026) Combining brain-wide activity imaging with electron microscopy reveals a distributed nociceptive network in the brain. bioRxiv 2025.09.25.678485. https://doi.org/10.1101/2025.09.25.678485 (preprint, in revision)

Dataset Contents

The viewer provides access to:

• The complete eFIB-SEM image volume of a Drosophila first instar larva CNS (sample 1099), 12 × 12 × 12 nm/voxel, served as N5
• A curated set of identified, named neuron reconstructions exported from the source project for hosting on VFB
• The associated neuron and annotation metadata

The hosted set is the curated, named-neuron export rather than the complete connectome; each skeleton carries its neuron-name annotations.

Features

This CATMAID instance enables:

• Browser-based visualisation of the eFIB-SEM image volume
• Navigation through the image stack
• Viewing of neuron reconstructions
• Neuron-name search
• API access for programmatic data retrieval (documentation: https://catmaid.readthedocs.io/en/stable/api.html): https://larva1099.catmaid.virtualflybrain.org/apis/

Citation Guidelines

When using this data, please cite:

1. The source dataset: Randel N, et al. (2026) Combining brain-wide activity imaging with electron microscopy reveals a distributed nociceptive network in the brain. bioRxiv 2025.09.25.678485. https://doi.org/10.1101/2025.09.25.678485

2. The CATMAID platform: Saalfeld S, Cardona A, Hartenstein V, Tomančák P (2009) CATMAID: collaborative annotation toolkit for massive amounts of image data. Bioinformatics 25(15): 1984-1986. https://doi.org/10.1093/bioinformatics/btp266

Maintenance & Support

This resource is archived, hosted, and maintained by Virtual Fly Brain (VFB - https://virtualflybrain.org) as part of its commitment to preserving and making accessible critical Drosophila neuroscience data resources.